The anticancer activity of A Squamosa seeds was evaluated at Regional Research Laboratory, Jammu. Cytotoxicity assay for assessment of growth inhibition was performed using Sulphorhodamine B (SRB) assay following National Cancer Institute (NCI) guidelines. The anti cancer assay was performed using sulphorhodamine B assay method on various cancer cell lines i.e. MCF-7, SiHa, HT, Ovcar-5, and HepG2. The maximum anticancer activity was observed with ovary cancer lines and minimum anticancer activity was observed in the adenocarcinoma cell lines.