Giardia lamblia and Entamoeba histolytica are two important parasites of the gastrointestinal tract causing gastroenteritis in human population. The present study is carried out to check the prevalence of these two parasites in the stream and open well water of district Hangu of Khyber Pakhtunkhwa 200/two hundred sample were examined through PCR which showed 26.5% (53/200) and 22.5% (45200) positive results for Giardia lamblia and E.histolytica respectively . The PCR product for Giardia lamblia was 163bp HSP (heat shock protein) and for Entamoeba histolytica was135bp small fragment of DNA.
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