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In this study, hollow fiber membrane (PAN,CA and
PSF) was hydrolyzed and grafted with antioxidants
such as conjugated linoleic acid (CLA)or heparin.
The effects of antioxidants grafting on the blood
coagulation, platelet aggregation, and oxidative
stress were evaluated using human blood. The
complete blood count (CBC) and coagulation time (CT)
was evaluated in vitro for the hemocompatibility.
Furthermore, the removal of bacterial endotoxin
(lipopolysaccharide, LPS) by antioxidant-bonding
membrane was measured with ELISA. In addition, the
production of reactive oxygen species (ROS) was
measured by chemiluminescence (CL) method to
evaluate the oxidative stress.
In vitro evaluation was carried out using mini-
hemodialyzer circulating with fresh porcine whole
blood to simulating kidney therapy. The dialysis
performance and hemocompatibility were estimated.
These results suggest that a antioxidant-grafting
membrane could offer protection for patients against
oxidative stress and would be helpful for reducing
the dosage of anticoagulant during hemodialysis.
PSF) was hydrolyzed and grafted with antioxidants
such as conjugated linoleic acid (CLA)or heparin.
The effects of antioxidants grafting on the blood
coagulation, platelet aggregation, and oxidative
stress were evaluated using human blood. The
complete blood count (CBC) and coagulation time (CT)
was evaluated in vitro for the hemocompatibility.
Furthermore, the removal of bacterial endotoxin
(lipopolysaccharide, LPS) by antioxidant-bonding
membrane was measured with ELISA. In addition, the
production of reactive oxygen species (ROS) was
measured by chemiluminescence (CL) method to
evaluate the oxidative stress.
In vitro evaluation was carried out using mini-
hemodialyzer circulating with fresh porcine whole
blood to simulating kidney therapy. The dialysis
performance and hemocompatibility were estimated.
These results suggest that a antioxidant-grafting
membrane could offer protection for patients against
oxidative stress and would be helpful for reducing
the dosage of anticoagulant during hemodialysis.