GREET enables a fast approach to an optimum Well-to-wheel analysis, while bringing new insights and improving the understanding of the nature of various biodiesel production processes. A simulation of Well-to-wheel analysis of Bio-diesel from soyabean is carried on GREET is discussed in this book. These simulation mentions about various steps involved in the analysis. It is followed by detailed tabulation of the analysis. Furthermore Life Cycle Analysis of Bio-diesel production from jatropha and micro algae is developed and discussed. In this book, Net Energy Ratio (NER)of these bio-diesel sources is calculated and a comparison with production of conventional diesel is made. Thus, the main aim of this book is to inform scholars about GREET and its effective use in industries as well as by research community and to assess the sustainability of Bio-diesel production from jatropha and micro algae and their comparison with conventional diesel.