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Implantation in humans involves cross talk between
an active blastocyst and receptive endometrium. The
role of the endometrial receptors in this complex
embryo-maternal interaction is still unclear. We
tested gene and protein expression of endometrial
receptors (Plexin-B1, c-Met and Progesterone
receptor A) and the effect of these receptors in
endometrial receptivity. Methods: Two endometrial
cell lines were used: HEC-1A and RL95-2 considered
as being of low and high receptivity, respectively.
Western blot and RT-PCR analysis were utilized to
study the receptor expression profile. The role of
endometrial receptors in endometrial receptivity was
studied by attachment and invasion assays of JAR
spheroids on endometrial cells. Different
manipulations of inhibition and stimulation of the
endometrial receptors were used.
Conclusion: Differential endometrial receptor
profiles are expressed during the receptivity
period. The attachment and invasion processes are
separately regulated. We suggest a biologically
functional role for PB1, PRA and the complex PB1-c-
MET in endometrial receptivity and in the attachment
process.
an active blastocyst and receptive endometrium. The
role of the endometrial receptors in this complex
embryo-maternal interaction is still unclear. We
tested gene and protein expression of endometrial
receptors (Plexin-B1, c-Met and Progesterone
receptor A) and the effect of these receptors in
endometrial receptivity. Methods: Two endometrial
cell lines were used: HEC-1A and RL95-2 considered
as being of low and high receptivity, respectively.
Western blot and RT-PCR analysis were utilized to
study the receptor expression profile. The role of
endometrial receptors in endometrial receptivity was
studied by attachment and invasion assays of JAR
spheroids on endometrial cells. Different
manipulations of inhibition and stimulation of the
endometrial receptors were used.
Conclusion: Differential endometrial receptor
profiles are expressed during the receptivity
period. The attachment and invasion processes are
separately regulated. We suggest a biologically
functional role for PB1, PRA and the complex PB1-c-
MET in endometrial receptivity and in the attachment
process.