Biotechnology is defined as the integrated use of scientific and engineering principles in the field of Biology. Bioengineering is a brand based engineering discipline that deals with biomolecular processes, product design, sustainability, immobilization and analysis of biological systems. Different microbes are used to convert cheap raw materials into economically valuable products. Generally amino acids are produced by various methods. Microbiological method is more advantageous amongst them. The use of immobilized whole cells reduce the process costs, increase the stability and maximize the utility. The co- immobilized cells can open up new possibilities of synergistic action. Increasing interest in commercial production of amino acids clearly reveals the many functions of amino acids in humans and animals. Novel methods of production are more economical. This book describes the production of L- Glutamic acid using microbes such as Brevibacterium saccharolyticum, B. roseum, and Escherichia coli Type 1, with free cells, immobilization and co-immobilization methods. Media design studies are carried out to enhance Glutamic acid yield with different nutrient enrichments.