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In vitro sperm activation is very important step in the laboratory technique that plays an important role in determination the outcome of assisted reproductive technologies like IUI. In addition, the sperm preparation technique used culture media and dependent according to properties of the semen parameters.Tamoxifen citrate is antiestrogen drug have antioxidant action which inhibited ROS production by break the oxidative chain reaction, thereby, reduce the oxidative stress. Also, prevention of lipid peroxidation and increased fluidity and flexibility which help the sperm to engage in membrane, fusion events associated with the fertilization.