RP-HPLC of peptides and proteins - Rotkaja, Oksana;Golushko, Jelena;Mekss, Peteris
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The separation of high-molecular compounds is very difficult, if possible at all, at isocratic conditions and gradient elution is needed. The theory of gradient elution for small molecules is well established; however its applications to reversed-phase gradient separations of biopolymers are not straightforward because of specific problems, such as slow diffusion, limited accessibility of the stationary phase for larger molecules, or possible sample conformation changes during the elution. High performance liquid chromatography has been used to investigate the reverse-phase chromatographic…mehr

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Produktbeschreibung
The separation of high-molecular compounds is very difficult, if possible at all, at isocratic conditions and gradient elution is needed. The theory of gradient elution for small molecules is well established; however its applications to reversed-phase gradient separations of biopolymers are not straightforward because of specific problems, such as slow diffusion, limited accessibility of the stationary phase for larger molecules, or possible sample conformation changes during the elution. High performance liquid chromatography has been used to investigate the reverse-phase chromatographic behavior of different proteins. By using a water/organic solvent/trifluoroacetic acid system the influence of experimental parameters was examined; chromatographic results from different stationary phases supports were comparable.
Autorenporträt
M.Sc Oksana Rotkaja has obtained her master's degree in analytical chemistry in 2010. She will obtain her PhD degree in analytical chemistry in 2016.She has worked in different projects in the field of analytical chemistry -chromatography. She in the author of several articles published in different journals.