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"Ones man's waste is another man's treasure." Marine sponge Clathria indica, are always dumped on the shores of Tamil Nadu as waste after fishing. They were collected from Thondi-Palk Strait shore. Secondary metabolites were isolated using Ethyl Acetate as the extractant from the crude extract, screened and studied for antagonistic activity against Urinary Tract pathogens Pseudomonas sp., Streptococcus sp. and Vibrio sp. isolated from UTI infected patient. There was an enormous antibacterial activity. DNA of the most potential hetero-bacterial strain of Clathria indica was analyzed and found to have high molecular weight than the control. This introduces a new antibacterial discovery against Urinary Tract pathogens.