The use of silver nanoparticles in nanomedicine and other silver nanoparticles combination like with biomaterials to reduce microbial contamination. In the field of nanomedicine and biomaterials, silver nanoparticles are used as an antimicrobial agent. The silver nanoparticles that could act as either (i) reducing agents stabilizing the silver nanoparticles or (ii) antimicrobial capping agents enhancing antimicrobial properties of silver nanoparticles. In this work, we reported the in vitro antimicrobial activity of silver nanoparticles synthesized by a green method using Garcinia cambogia fruit extract and were tested against one bacterial strain (Escherichia coli) and one fungus (Candida albicans) of clinical interest using the agar well diffusion method and Biogenic silver nanoparticles showed a higher inhibitory effect against Escherichia coli than Candida albicans.