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The use of prescribed therapeutic antibiotics has met with some limitations such as initiation of allergic reactions in individuals, elimination of normal flora and ineffective action against several emerging antibiotic resistant microorganisms. These, among other constrains, limit the application of antibiotics in food preservation, clinical utilization and other situations. Consequently, there has been a growing interest in developing safe and effective microbial substitutes like bacteriocins. This book, however, provides researchers a typical stepwise approach to isolate soil bacteria (especially, species belonging to Pseudomonas and Bacillus genera) with potentials to produce bacteriocins and bacteriocin-like extracellular metabolites, as well as procedures to characterize such excreted metabolites.