Health care workers (HCWs) play a vital role in preventing hospital-acquired infections such as methicillin-resistant Staphylococcus aureus (MRSA), failure to adhere to standard precautions may lead to the spread of multidrug-resistant organisms such as MRSA and increase the morbidity and mortality of patients. The main objective of the study is to assess knowledge, attitudes, and practices of HCW's in West Bankgovernmental- hospitals toward prevention strategies for MRSA.