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The present investigation entitled, "Assessment of chemical and biological parameters in sorghum-wheat cropping sequence under long term fertilization" was under taken during the year 2007-08 with a view to study the long term changes in soil fertility. The long term experiment was initiated at Central Research Station, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola in the year 1988. The twelve treatments replicated four times in Randomized Block Design which comprised of NPK levels with and without FYM, Sulphur and Zinc.