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Today Coordination Chemistry comprises a large body of Inorganic Chemistry research; it has fascinated and inspired the chemists all over the world. There is an ever increasing academic, commercial and biochemical interest on the metal complexes of organic chelating ligands. This has resulted in the emergence of applied fields like Organometalic Chemistry, Homogeneous Catalysis and Bioinorganic Chemistry. Between all of the chelating ligands, Isatin-hydrazone Ligands have attracted the attention of Chemists due to the ease of preparation and complexation.So, it Is important to create novel isatin derivatives for emerging drug targets. In this work we aim to design some Ni(II) complexes with newly synthesized Isatin-bishydrazones ligands, Testing their in-vitro antibacterial and antifungal activity against many types of bacteria and anti fungal cultures, which are common contaminants of the environment in Egypt,and also investigate their interaction with (CT-DNA) using UV-visible spectroscopy, and hydrodynamic technique under physiological conditions (at stomach (4.7) and blood (7.4) pH and at body temperature (37 0C) ).