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This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. Written by experts in this field, the text provides theoretical consideration, as well as practical approaches to in situ PCR. Organized in a user-friendly two-column format, it provides step-by-step protocols, preparation of reagents and solutions, and appropriate controls to allow the researcher to perform and optimize these sensitive and powerful techniques.…mehr

Produktbeschreibung
This book covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two new approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. Written by experts in this field, the text provides theoretical consideration, as well as practical approaches to in situ PCR. Organized in a user-friendly two-column format, it provides step-by-step protocols, preparation of reagents and solutions, and appropriate controls to allow the researcher to perform and optimize these sensitive and powerful techniques.

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, B, BG, CY, CZ, D, DK, EW, E, FIN, F, GR, HR, H, IRL, I, LT, L, LR, M, NL, PL, P, R, S, SLO, SK ausgeliefert werden.

Autorenporträt
Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.