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Sugarcane is one of the major sugar crops of the world. In Pakistan, it occupies a significant position in the agricultural economy. The present investigations were carried out to examine the genetic variability and contribution of some morphological characters for cane yield and response of genotypes of sugar cane toward callus formation, regeneration and screening for virus. The results pertaining to analysis of variance elucidated highly significant differences among the accessions for all the traits. The tissue culture study revealed that at different treatment over all performance of HSF-247 was better for callus induction and genotype HSF-242 performed best at 3mg/l of 2, 4-D level. All the investigation in the correlation study proved quite successful because a handful increase in cane weight and plant height is possible as they provide information regarding a suitable combination of characters with their maximum influence on the enhancement of cane weight and plant height. The clone will be selected with such combinations of characters which to give maximum improvement in their cane yield.