Plants synthesize certain chemicals for the sake of protection against the organisms which are harmful to them or for the sake of competition for the natural resources. These chemicals are generally categorized as secondary metabolites. The secondary metabolites are equally beneficial for human being too as these are medicinally important. Literature survey shows the enhancement of plumbagin content was enhanced by micro-propagation through hairy root culture. The plumbagin was also found to be increased through cell culture technique by elicitation. In the present investigation attempts have been made to enhance plumbagin content in Plumbago zeylanica by entirely different method i.e. in vivo elicitation through bacterial treatment.