This practical manual is on Basic techniques in Biotechnology provides activities that are well standardized for large as well as small class groups of the post-graduate and undergraduate biotechnology students. The analytical techniques mentioned in manual form the central building pillar in any biotechnological approach around which, most of the recent research development revolves. This manual accentuates basic experimental, observational, investigatory and interpretational skills in students, which is consummate of acquiring laboratory skill. Comprehensive safety notes and the principle/rationale behind each experiment are very well laid out. As reflected in the title, the authors are sure that this manual would bring out the fundamental principles in biotechnological experiments and would certainly help the new generation biologists.