Kinetic Study Of Lactic Acid Production From Lactobacillus Cells
Taguchi Optimization, Lactic Acid, Aqous Two Phase System, Mannitol, Metabolic Engineering
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Present study is on Kinetic studies of lactic acid production from lactobacillus cells The thesis contains following parts; Screening of microorganism for lactic acid production, Optimization of medium components, Production kinetics, Strain improvements, and lactic acid recovery. Screening of microorganism was done on the basis of lactic acid production, substrate utilization, and cell mass formation by three species L. helveticus, L. delbrueckii and L. casei. Among three species studied L. delbrueckii was found to utilize maximum (88%) glucose and (90%) sucrose and thus produces 82 g/l lacti...
Present study is on Kinetic studies of lactic acid production from lactobacillus cells The thesis contains following parts; Screening of microorganism for lactic acid production, Optimization of medium components, Production kinetics, Strain improvements, and lactic acid recovery. Screening of microorganism was done on the basis of lactic acid production, substrate utilization, and cell mass formation by three species L. helveticus, L. delbrueckii and L. casei. Among three species studied L. delbrueckii was found to utilize maximum (88%) glucose and (90%) sucrose and thus produces 82 g/l lactic acid with cell mass 10 and 11 g/l. Maximum yield obtained on increasing sucrose concentration from 10% to 15%, was 0.89 g/g while on increasing sucrose up to 20% results in decrease of yield (0.61 g/g). As sucrose is present in cane molasses which is cheap and widely available in India, therefore, L. delbrueckii was used with cane molasses for lactic acid production. Media optimization andlactic acid purification was done by Taguchi methods ;strain improvement was done by UVmutagenesis.
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