Silas G. Villas-Boas, Jens Nielsen, Jorn Smedsgaard

Metabolome Analysis

An Introduction
Mitarbeit: Villas-Boas, Silas G.; Nielsen, Jens; Smedsgaard, Jorn

• Gebundenes Buch

Produktbeschreibung

A concise, interdisciplinary introduction to metabolome analysis

This practical reference focuses on the principles of metabolome analysis and treats metabolomics as a field of its own, rather than as just an additional analytical tool in science. Part I guides readers through the primary steps in metabolite analysis, beginning with an overview of the terminology and the basic concepts of cell metabolism, and the dynamics of biochemical reactions and metabolite turnover. It then discusses the most common methodologies for sample preparation, sample techniques, detection and identification methods, analytical tools (including liquid and gas chromatography as well as mass spectrometry), and data analysis.

Part II illustrates the applicability of metabolomics and discusses specific peculiarities and requirements of metabolomics in certain groups of organisms. It reviews successful cases of metabolome analysis with chapters on:
_ Yeast metabolomics
_ Specializedsampling devices for microbial metabolomics
_ Major achievements in plant metabolomics
_ Metabolomics in the classification of filamentous fungi
_ Metabolomics applied to humans and other mammals

This is the definitive, core reference on metabolome analysis for research scientists in biochemistry and analytical chemistry, as well as academics, researchers, and technicians in the fields of functional genomics and metabolic engineering. It is also a practical text for scientists working to discover metabolites from natural sources and for graduate students in a variety of courses.

Produktdetails

• Wiley-Interscience Series on Mass Spectrometry
• Verlag: Wiley & Sons
• Artikelnr. des Verlages: 14674344000
• 1. Auflage
• Seitenzahl: 330
• Erscheinungstermin: 1. Februar 2007
• Englisch
• Abmessung: 242mm x 164mm x 22mm
• Gewicht: 600g
• ISBN-13: 9780471743446
• ISBN-10: 0471743445
• Artikelnr.: 22528598

Autorenporträt

SILAS G. VILLAS-BÔAS, PHD, is a Research Scientist at AgResearch Limited in New Zealand. UTE ROESSNER, PHD, is a Post-Doctoral Fellow at the Australian Centre for Plant Functional Genomics at the University of Melbourne, Australia.? MICHAEL A. E. HANSEN, PHD, is a Post-Doctoral Fellow at BioCentrum-DTU, Technical University of Denmark. JØRN SMEDSGAARD, PHD, is an Associate Professor at the Center for Microbial Biotechnology, BioCentrum-DTU?at the Technical University of Denmark. JENS NIELSEN, PROFESSOR, Dr. techn., PHD, is the Director of the Center for Microbial Biotechnology at the Technical University of Denmark.

Inhaltsangabe

PREFACE. LIST OF CONTRIBUTORS. PART I: CONCEPTS AND METHODOLOGY. 1
Metabolomics in Functional Genomics and Systems Biology. 1.1 From genomic
sequencing to functional genomics. 1.2 Systems biology and metabolic
models. 1.3 Metabolomics. 1.4 Future perspectives. 2 The Chemical Challenge
of the Metabolome. 2.1 Metabolites and metabolism. 2.2 The structural
diversity of metabolites. 2.3 The number of metabolites in a biological
system. 2.4 Controlling rates and levels. 2.5 Metabolic channeling or
metabolons. 2.6 Metabolites are arranged in networks that are part of a
cellular interactome. 3 Sampling and Sample Preparation. 3.1 Introduction.
3.2 Quenching--the fi rst step. 3.3 Obtaining metabolites from biological
samples. 3.4 Metabolites in the extracellular medium. 3.5 Improving
detection via sample concentration. 4 Analytical Tools. 4.1 Introduction.
4.2 Choosing a methodology. 4.3 Starting point--samples. 4.4 Principles of
chromatography. 4.5 Chromatographic systems. 4.6 Mass spectrometry. 4.7 The
analytical work-fl ow. 4.8 Data evaluation. 4.9 Beyond the core methods.
4.10 Further reading. 5 Data Analysis. 5.1 Organizing the data. 5.2 Scales
of measurement. 5.3 Data structures. 5.4 Preprocessing of data. 5.5
Deconvolution of spectroscopic data. 5.6 Data standardization
(normalization). 5.7 Data transformations. 5.8 Similarities and distances
between data. 5.9 Clustering techniques. 5.10 Classifi cation techniques.
5.11 Integrated tools for automation, libraries, and data evaluation. PART
II: CASE STUDIES AND REVIEWS. 6 Yeast Metabolomics: The Discovery of New
Metabolic Pathways in Saccharomyces cerevisiae. 6.1 Introduction. 6.2 Brief
description of the methodology used. 6.3 Early discoveries. 6.4 Yeast
stress response gives evidence of alternative pathway for glyoxylate
biosynthesis in S. cerevisiae. 6.5 Biosynthesis of glyoxylate from glycine
in S. cerevisiae. 7 Microbial Metabolomics: Rapid Sampling Techniques to
Investigate Intracellular Metabolite Dynamics--An Overview. 7.1
Introduction. 7.2 Starting with a simple sampling device proposed by
Theobald et al. (1993). 7.3 An improved device reported by Lange et al.
(2001). 7.4 Sampling tube device by Weuster-Botz (1997). 7.5 Fully
automated device by Schaefer et al. (1999). 7.6 The stopped-fl ow technique
by Buziol et al. (2002). 7.7 The BioScope: a system for continuous-pulse
experiments. 7.8 Conclusions and perspectives. 8 Plant Metabolomics. 8.1
Introduction. 8.2 History of plant metabolomics. 8.3 Plants, their
metabolism and metabolomics. 8.4 Specifi c challenges in plant
metabolomics. 8.5 Applications of metabolomics approaches in plant
research. 8.6 Future perspectives. 9 Mass Profi ling of Fungal Extract from
Penicillium Species. 9.1 Introduction. 9.2 Methodology for screening of
fungi by DiMS. 9.3 Discussion. 9.4 Conclusion. 10 Metabolomics in Humans
and Other Mammals. 10.1 Introduction. 10.2 A brief history of mammalian
metabolomics. 10.3 Sample preparation for mammalian metabolomics studies.
10.4 Sample analysis. 10.5 Applications. 10.6 Future outlook. INDEX.

Rezensionen

"Overall, this book provides a great introduction to metabolomicsand at a retail price of approximately $75, it has great value. Theintended audience for this book includes new metabolomicspractitioners and undergraduate/graduate students." (J Am Soc MassSpectrom, 2007)