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Propolis is a resinous mixture collected from trees by the Apis mellifera bee, which uses as a building insulating material in the beehive as well as for keeping it in good health. It has important pharmacological properties and it can be used for a wide range of purposes as anti-cancer, antioxidant, immune system stimulant, and bacteriostatic and bactericidal agent, among many other uses.The distinct chemistry of propolis from different origins leads to the expectation that the biological properties of different propolis types will be dissimilar. This study aims to determine the chemical composition and biological activity of propolis propolis taken from several areas of Java. This study is divided into several stages, among others: a) testing the chemical composition of propolis is taken from Sukabumi (West Java), Batang (Central Java) and Lawang (East Java), the parameters are analyzed phytochemical screening, GC-MS analysis, the determination of levels of flavonoids, TLC densitometry, b) cytotoxic assay using MCF-7 and T-47D cell lines, anti-plasmodium and antimycobacterium, c) in vivo anticancer testing using strain C3H mice and acute and subacute toxicity test.