Canine parvovirus infection, a highly contagious disease of canines is prevalent all over the world, mainly because the virus can survive in adverse environmental conditions for a long time. Natural CPV-2 infection has been reported in domestic dogs, bush dogs, cats, coyotes, bears and wolves. The disease is clinically characterized by severe vomiting, pyrexia, anorexia and diarrhoea leading to fatal dehydration as well as myocarditis particularly in young pups. This research focuses on molecular epedemialogy, isolation and characterization of canine parvo virus.