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The assignment of the sites of modification by
different carcinogens within a target sequence is a
very important topic in cancer etiology and a
critical part in the analysis of DNA adducts is to
determine the site and frequency of modification on
the DNA strands in order to better understand the
relationship between the chemical behavior of
different carcinogens and mutation hot spots. The
initial focus was on the analysis of DNA adducts
as monomeric units , i.e., nucleosides or
nucleotides. In order to correlate the
adducts formation and gene sequence, it is necessary
to have a fast and accurate methodology for
structural characterization of oligonucleotide-
carcinogen adducts from in vitro and in vivo sources
using on-line separation coupled with MS or tandem
MS. The aim of this book s research has been to
develop HPLC-MS methodology for the analysis of DNA
adducts in the form of oligonucleotides. To achieve
this goal, a thorough literature review was
conducted and a systematic approach with carefully
designed experiments was carried out along with a
discussion on presents the future prospects of the
research.
different carcinogens within a target sequence is a
very important topic in cancer etiology and a
critical part in the analysis of DNA adducts is to
determine the site and frequency of modification on
the DNA strands in order to better understand the
relationship between the chemical behavior of
different carcinogens and mutation hot spots. The
initial focus was on the analysis of DNA adducts
as monomeric units , i.e., nucleosides or
nucleotides. In order to correlate the
adducts formation and gene sequence, it is necessary
to have a fast and accurate methodology for
structural characterization of oligonucleotide-
carcinogen adducts from in vitro and in vivo sources
using on-line separation coupled with MS or tandem
MS. The aim of this book s research has been to
develop HPLC-MS methodology for the analysis of DNA
adducts in the form of oligonucleotides. To achieve
this goal, a thorough literature review was
conducted and a systematic approach with carefully
designed experiments was carried out along with a
discussion on presents the future prospects of the
research.