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rtation results showed that the test extract recovers the blood parameter in curative and preventive study, while mechanistic study showed protection of RBC membrane when exposed to ruinous substances. Thus, present finding affirm assentation of this plant in folk medicine for the management of hemolytic anemia.Traditional oral report indicates that fruits of opuntia ficus indica are used in the treatment of anemia in India. It is cheaper and widely available plant. The claims reported in favour of opuntia ficus indica are not substained by modern scientific studies. For this purpose, the present study was carried out to validate effect of dehydrated water extract of opuntia ficus indica on experimentally-induced hemolytic anemia in rats. In mechanistic study, the protective actions of test extract on red blood cells against hypotonic solution, verapamil and calcium free medium was evaluated. Present book results showed that the test extract recovers the blood parameter in curative and preventive study, while mechanistic study showed protection of RBC membrane when exposed to ruinous substances. Thus, present finding affirm assentation of this plant in folk medicine for the management of hemolytic anemia.