The electrostatic force between bacteria and NPs must take into consideration as samples with a negative hall coefficient have a bactericidal effect on Gram (+) bacteria, in contrast to samples with a positive hall coefficient. In consequence, it is possible to expect the antimicrobial properties from the gradient in electron energy or a gradient in the electrical potential where this mechanism involves a basic excitation process as an interaction between electrons and the lattice vibrations of the crystal. The band theory must take into consideration as it may give a qualitative result in the analysis of the antimicrobial properties. Initial bacterial adhesion is recognized to be the essential step for biofilm formation. As the material is negatively charged, this may reduce bacteria attachment and delay biofilm formation. Material with positively charged surface attracts bacteria and therefore this electrostatic force disrupts surface growth. Most implantation failure comes from bacterial infection, positively charged surfaced may control this drawback.
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