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Objective of the study is to check the sterility of nelaton tube and optimize Gamma radiation dose for the elimination of microbial contamination.Medical devices are a reservoir for the embellishment of bacteria, viruses, fungi, parasite and prions, all of which are a leading cause of Hospital Acquired Infections. The information provided will help to raise the healthcare standards in Pakistan and decrease the institutional costs associated with improperly sterilized nelaton tube.