In the present work, screened 113 germplasm lines and isolated genotypes CO3, Arka Meghali (large fruit genotypes) and EC-521038 and EC-24296 (small fruit genotypes) promising for heat stress and subjected for induced mutagenesis using gamma rays and EMS. Fixed LD50 value, at 350 Gy and 0.30% for EMS respectively. Higher frequency of chlorophyll mutants (0.60%) and viable mutant frequency (6.25%) was observed in EMS treating Arka Meghali followed by CO3 (0.35% & 5.14%). The viable mutants MC-14, MC-27, MC-20, MC-83, MC-46, MC-136, MA-41, MA-18, ME-516, ME-518, ME-12, ME-519, ME-22, ME-504, M-18, ME-217, ME-212, ME-220, ME-221, ME-230 and M-6 had larger fruit size (>60 g), high yield (>1900-2200g/plant) and high lycopene content (7-7.6 mg/ 100g) scoping to develop commercial varieties after attaining homogeneity in M4 and subsequent generations. The mutant MC-62 and MA-47 had high yield with yellow fruit colour, and ME-13, ME-16, ME-30, M-3 and M-23 mutants having high lycopene content with low yield potential, have bright scope for development of breeding lines for future studies related to fruit ripening mechanism in tomato.