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The present study was conducted in the laboratory of plant tissue culture, Department of Biology, College of Science, University of Al- Mustansiriyah, in order to increase the production of some secondary metabolic compounds in callus induce from leave and shoot tips of Olea europaea L. in vitro.Secondary metabolites were estimated quantitatively and qualitatively using high performance liquid chromatography HPLC and compared with those in the mother plant. Some elicitors were used including Poly Ethylene Glycol (PEG)and silver nano particles AgNO3 NPs. Results showed that concentrations of AgNO3 NPs caused highly significant production in most of the secondary metabolites (Glycoside compounds) of callus for Olea europaea L. Results showed that concentrations of PEG caused highly significant production of the secondary metabolites (Phenolic compounds) of callus for Olea europaea L.