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Quantitative, descriptive, cross-sectional study was conducted to determine the level of knowledge and extent of Computer Vision Syndrome (CVS) among computer users in the Securities and Exchange Commission (SEC), Abuja, Nigeria. Structured questionnaires were administered to 100 computer users aged between 18 and 40 years. The study findings revealed that 40 respondents (40%) were aware of CVS and 27 (27%) of them had knowledge of the disorder. 74 (74%) of the respondents experienced at least one symptom of CVS. Headache and eyestrain were the most common symptom of CVS among the population. The study also revealed that the internet (accounting for 50%) was the major source of information about CVS awareness. The study concluded that 27% knowledge level is too low and much emphasis is needed to educate the people at risk of CVS. Key terms: Computer Vision Syndrome; knowledge; awareness; workplace; computer users; glare; headache; eyestrain; presbyopia; ergonomics.