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This monograph will have practical use to those who are interested to establish embryonic stem cells laboratory. Embryonic stem cells are one of the emerging areas in reproductive biotechnologies in buffalo, which could have enormous agricultural and biomedical applications. Besides its immense potential in humans, the stem cell technology has proved to be a boon for farm animals since promising results have been obtained in the fields of gene targeting, cloning, transgenesis, chimera formation and differentiation into germ cells (sperm and oocytes). This book mainly deals with the isolation,…mehr

Produktbeschreibung
This monograph will have practical use to those who are interested to establish embryonic stem cells laboratory. Embryonic stem cells are one of the emerging areas in reproductive biotechnologies in buffalo, which could have enormous agricultural and biomedical applications. Besides its immense potential in humans, the stem cell technology has proved to be a boon for farm animals since promising results have been obtained in the fields of gene targeting, cloning, transgenesis, chimera formation and differentiation into germ cells (sperm and oocytes). This book mainly deals with the isolation, culture, characterization and differentiation of embryonic stem cells generated from in vitro fertilized embryos in buffalo and grown on different feeder cells. It will also give the overview of preparation of feeder cells, cryopreservation, in vitro fertilization and parthenogenetic activation.
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Autorenporträt
Dharmendra Kumar lavora come dottorando di ricerca presso la School of Medical and Allied Sciences, Galgotias University, Greater Noida, India ed è autore di oltre 20 pubblicazioni in diverse riviste internazionali e nazionali di fama. Ha pubblicato 05 brevetti e lavora come redattore e revisore in molte riviste diverse. Ha conseguito il Master in Farmacia.