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Gymnema sylvestre is a medicinal plant with huge demand in pharmaceutical and in medicinal fields because of its bioactive compounds. Medicinal plants have been recognized as repository of fungal endophytes with novel metabolites of medicinal importance.Successful inoculation with fungal endophytes and endomycorrhiza (VAM) will result in enhanced growth and improved production and accumulation of Gymnemic acid in Gymnema sylvestre. These fungal endophytes will serve as an alternate source of novel metabolites of pharmaceutical importance.