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The aim of the book is to investigate plasma free amino acids profile as a possible diagnostic/prognostic factor in hepatocellular carcinoma patients. This study included 71 Hepatocellular cacinoma patients, and 30 chronic HCV patients in addition to 30 healthy controls. Laboratory investigations including; complete blood picture, liver function tests, serum alpha fetoprotein, hepatitis viral markers. The book showed a statistically significant increase of methionine, tyrosine, ornithine, citrulline, glycine, phenylalanine, alanine, glutamate, proline, and arginine and decrease of valine, aspartate, leucine/isoleucine, BTR and fischer ratio in hepatocellular carcinoma compared to control group. Also, it indicated that there was a statistically significant correlation between BTR and platelets, alanine transaminase, albumin, bilirubin and alpha fetoprotein and significant differences in BTR among Child-Pugh A, B and C. Our results suggested that the plasma free amino acids profileis considered valuable for diagnosis and nutritional care in cancer patients.Furthermore, BTR reflects the pathological liver background with a high degree of correlation to other liver functional markers.