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Global research focuses on sustainable development and the demand for innovative clean technologies, yet revisiting the natural potential could represent available solution for identifying and designing new pharmacological agents from renewable resources. Many studies exposed those endophytic bacteria contribute effectively in enhancing plant growth, the resistance of biotic and abiotic tensions, detoxification of destructive compounds, bioactive compounds production, bio-products or to modify and introduce useful bacteria into the plant Phyto-microbiome for agricultural purposes. From other side, natural chemicals are very important as they are integrated into several industries such as pharmaceutical, cosmetic, agro-industrial, and nutritional products. the present book aims at isolation and characterization of endophytic bacteria from the medicinal plant Senecio glaucus that collected from different habitats from Egypt and studying their function properties. Also, evaluation of the different of the plant bioactive compounds and their biological activities for each habitat.