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Helicobacter species pylori represent one of the medically prominent and most common infections in the world. Contamination with this microbe has set as a causal factor in the development of gastritis, peptic ulcer, and gastric neoplasia. Consequently, prompt diagnosis is essential. Therefore, regarding all facts about the strong correlation between H. pylori and different upper gastrointestinal lesions, it is very crucial to specify the presence of this bacteria in gastric biopsy reports as it has an important role in the therapeutic implications too. Since, over years pathologists have sought different reliable methods for detecting H. pylori in biopsy specimens, including special stains, immunohistochemistry (IHC), polymerase chain reaction (PCR), and lately, in situ hybridization.