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Leishmania major infectious diseases of trouble for the people of the world. So to control the disease and its spread in other areas have done a lot of research, especially research on the synergistic effects to treatment plants. Leishmaniasis is one of the major problems in health systems. Due to the side effects of medications used to treat these diseases and people also tend to use herbal medicines. This study aimed to investigate the effect of ethanol extracts of three plants, yarrow, wormwood and walnut leaf extracts on Leishmania major and mixed three synergistic effects in vitro In vitro study was done. Microbiology, Parasitology, Botanical medicines, The epidemiology of infectious diseases in the world and Iran.