A number of excellent and well-established textbooks are available to support study of microbiology however, such books are mostly over 1000 pages in length, beautifully illustrated in colour, but rather expensive and in effective but complex language and terminology. This can hamper the understanding of some students. This book in no way seeks to replace or compete with such texts, it is directed rather towards the students, who require a text that is less detailed, less comprehensive, easy explanation and less expensive. To embark on this study, it will be necessary for the students to learn how to handle cultures in such a way that they are not contaminated or inadvertently dispersed throughout the classroom. This involves learning aseptic techniques and practicing preventive safety measures. Above all, I have tried to explain concepts or mechanisms behind each method, with an objective of easier understanding of methods and techniques. It is our wish that the present text will give the attentive enthusiastic reader a clear perceptive of sometimes-complex issues, whilst avoiding over-simplification.