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Produktbild: Molecular Methods for Evolutionary Genetics
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Molecular Methods for Evolutionary Genetics

Aus der Reihe Methods in Molecular Biology

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

29.09.2011

Abbildungen

XVI, 81 illus., 4 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Virginie Orgogozo + weitere

Verlag

Humana Press

Seitenzahl

503

Maße (L/B/H)

26/18,3/3,3 cm

Gewicht

1133 g

Auflage

2011

Sprache

Englisch

ISBN

978-1-61779-227-4

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

29.09.2011

Abbildungen

XVI, 81 illus., 4 illus. in color., schwarz-weiss Illustrationen, farbige Illustrationen

Herausgeber

Verlag

Humana Press

Seitenzahl

503

Maße (L/B/H)

26/18,3/3,3 cm

Gewicht

1133 g

Auflage

2011

Sprache

Englisch

ISBN

978-1-61779-227-4

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Molecular Methods for Evolutionary Genetics

  • Part I. Characterizing the Genome

     

    1. Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei

                Emily E. Hare and J. Spencer Johnston

     

    2. Chromosome Analysis in Invertebrates and Vertebrates

                David M. Rowell, Shu Ly Lim and Frank Grutzner

     

    3. Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries

                Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter

     

    4. Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones

                Mike A. Quail, Lucy Matthews, Sarah Sims, Christine Lloyd, Helen Beasley, and Simon W. Baxter

     

     

    Part II. Targeting Regions of the Genome

     

    5. Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing

                Jeffrey M. Good

     

    6. Accessing the Transcriptome: How to Normalize mRNA Pools

                Heiko Vogel and Christopher W. Wheat

     

    7. Transcriptome Sequencing Goals, Assembly, and Assessment

                Christopher W. Wheat and Heiko Vogel

     

    8. Rapid Retrieval of DNA Target Sequences by Primer Extension Capture

                Adrian W. Briggs

     

    Part III. Measuring Genetic Diversity

     

    9. SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing

                Paul D. Etter, Susan Bassham, Paul A. Hohenlohe, Eric Johnson, and William A. Cresko

     

    10. DNA Microarray-based Mutation Discovery and Genotyping

                David Gresham

     

    11. Genotyping with Sequenom

                Martina Bradić, João Costa, and Ivo M. Chelo

     

    12. Isolating Microsatellite Loci: Looking Back, Looking Ahead

                José A. Andrés and Steven M. Bogdanowicz

     

    13. Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector

                Amy A. Caudy

     

    Part IV. Obtaining Candidate Gene Sequences

    14. Identification of Homologous Gene Sequences by PCR with Degenerate Primers

                Michael Lang and Virginie Orgogozo

     

    15. Characterizing cDNA Ends by Circular RACE

                Patrick T. McGrath

     

    16. Identification of DNA Sequences that Flank a Known Region by Inverse PCR

                Anastasios Pavlopoulos

     

    Part V. Analyzing Candidate Gene Transcripts

    17. Quantification of Transcript Levels with Quantitative RT-PCR

                Karen L. Carleton

     

    18. Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences

                Patricia J. Wittkopp

     

    19. Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern

                Ryo Futahashi

     

    20. Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning            

                Scott Rifkin

     

    Part VI. Testing Candidate Genes and Candidate Mutations

     

    21. Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution

                Mark Rebeiz and Thomas M. Williams

     

    22. Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution

                Jay F. Storz and Anthony J. Zera

     

    23. Making Reporter Gene Constructs to Analyze Cis-regulatory Elements

                José Bessa and José Luis Gómez-Skarmeta

     

    24. PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker’s Yeast

                Erik C. Andersen

     

    25. Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species Transgensis

                Radoslaw Kamil Ejsmont, Maria Bogdanzaliewa, Kamil Andrzej Lipinski, and Pavel Tomancak

     

    26. Recombination-mediated Genetic Engineering of Large Genomic DNA Transgenes

                Radoslaw Kamil Ejsmont, Peter Ahlfeld, Andrei Pozniakovsky, A. Francis Steward, Pavel Tomancak, and Mihail Sarov

     

    27. Overlap Extension PCR: An Efficient Method for Transgene Construction

                Matthew D. Nelson and David H. A. Fitch

     

    28. Gene Knockdown Analysis by Double-stranded RNA Injection

                Benjamin N. Philip and Yoshinori Tomoyasu