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Groundwater forms the major source of drinking water in the rural areas of most of the developing nations in the world. Presence of high concentration of nitrate in groundwater is a major problem in many countries as it causes health related problems. In an agro-economy based rural areas, the nitrate contamination is rampant and much attention has not been drawn towards this anthropogenic pollution. The present study emphasizes the objective of determining the causes for higher nitrate concentration in groundwater and health implications in parts of Anantapur District, Andhra Pradesh, India.