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Bio surfactants are biological surface active agents capable of reducing interfacial tension between liquids, solids and gases. There by allowing them to mix and disperse readily in water or other liquids.Bio surfactants are interacts with the phase boundary in heterogeneous system. Then on polar "tail" is typically a hydrocarbon chain whereas the polar "head" appear s in many different varieties such as carbohydrates, amino acids or phosphates. Surfactants are used for a wide variety of applications in households,industry and agriculture .they are extensively used in cleaning applications and as a formulation aid to promote solubilization,emulsification and dispersion of other molecules in products ranging from Chemicals,cosmetics,detergents,food,textiles and pharmaceuticals. Surfactants are molecules that intervene in hearly aspect of human daily life.This book is an attempt to explore various methods to study biosurfactants. This book would be very useful to researchers,R&D labs upcoming students and scientists who wish to explore microbial diversity and bioprospection.