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Plants are the foundation of all life that exists on the planet Earth. Plant cells contain plastids, which are double-membrane cell organelles. Plastids perform the function of manufacturing and storing food. These cell organelles can be classified into three types, namely, chloroplasts, chromoplasts, and leucoplasts. Chloroplasts are the organelles responsible for food production in plants. Stroma, inner membrane, outer membrane, thylakoid membrane, and intermembrane space are specific parts of chloroplasts. There are various functions of chloroplasts, including photosynthesis, pathogen defense, amino acid synthesis, carbon fixation and G3P synthesis. Photosynthesis is the main function of chloroplasts, which involves the conversion of light into chemical energy in order to produce food in the form of sugars. This book outlines the basic concepts and applications of chloroplast in detail. It consists of contributions made by international experts. This book aims to serve as a resource guide for students and experts.