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Thin films of PEG embedded with silver nanoparticles were prepared using an electrospray deposition technique (ESD). The antimicrobial activity of the prepared Ag/PEG nanocomposites towards some pathogenic microorganisms such as gram positive bacteria, gram negative bacteria and fungi was elucidated and indicated that Ag/PEG nanocomposites have a selective antimicrobial action. This demonstrates that the composite films might be employed as environmentally friendly and antimicrobial surface in the biomedical applications. Therefore, it could be useful for drinking water treatment, disinfection.