The thermoluminescence (TL) studies of calcite mineral have resulted in the development of a wider range of applications in ceramic industry field. The TL glow curve shapes of minerals changes as a result of crystal purity, radiation dose, and thermal history previous the irradiation. In this study, the TL dose response of natural calcite samples of unannealed and annealed at different temperatures has been studied. The calcite samples that were collected from Elaz g-Ag n city in Turkey exhibit two main TL peaks following beta-irradiation. The samples were annealed at 600, 700, 800, 900, 1000 oC from 15 minutes to 240 minutes and then they were irradiated at room temperature with a point beta source for different time durations to study TL dose response curve. It was observed that the annealings at 900 and 1000 oC have high prononuced effects on the dose response curve of natural calcite.