Most often forensic test for Identification of Biological material are destructive in nature during the search of crime scene. At crime scene which sample have to pack which have to left, it is decided either by seen naked eye or torch/UV light. we have to practice non- destructive technique for presumptive identification. technique like use of light at specific wavelength and combination of filter with light. Given method is helpful for crime scene search and help to located and visualize biological stain. Resultant will be easy in sample lifting. Highly useful for searching biological stain at crime scene.