Filter Binding Assay
Biochemistry, Chemistry, Binding constant, Adenosine triphosphate
Herausgegeben: Cristóbal, Barnabas
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In biochemistry or chemistry, one of the ways to learn about an interaction between two molecules is to determine the binding constant, which is a number that describes the ratio of unbound and bound molecules. This information reveals the affinity between the two molecules and allows prediction of the amount bound given any set of initial conditions.In order to measure a binding constant, one must find a way to measure the amount of complex formed over a range of sta...
Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. In biochemistry or chemistry, one of the ways to learn about an interaction between two molecules is to determine the binding constant, which is a number that describes the ratio of unbound and bound molecules. This information reveals the affinity between the two molecules and allows prediction of the amount bound given any set of initial conditions.In order to measure a binding constant, one must find a way to measure the amount of complex formed over a range of starting concentrations. This can be achieved by "labeling" one of the species with a fluorescent, or in this case, a radioactive tag. The DNA is "labeled" by the addition of radioactive Phosphate derived from Adenosine Triphosphate.A filter binding assay is a simple way to quickly study many samples. It measures affinities between two molecules (often protein and DNA) using a filter
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