The manuals divided into three main sections. First section deals general introduction and laboratory safety. Second section deals with qualitative analysis of carbohydrate and amino acid. The third section deals with quantitative analysis along with biomolecules preparations.The present script is just a compilation of facts and interpretation from different sources. The author does not claim the originality of the subjects. The present manual is the author's understandings of the various techniques described and are fully responsible for the errors and misinterpretations.