Going beyond step-by-step descriptions of how to perform techniques, Methods in Neuroendocrinology examines the questions being asked in the field, including what problems need to be solved and what the limits of interpretation are. The experimental approaches described in this book merge endocrine and neuroscientific research and offer insight into the many disciplines involved. Much more than a "cookbook", this is a resource for every researcher who wants to pursue new areas of inquiry in the science of neuroendocrinology.
Going beyond step-by-step descriptions of how to perform techniques, Methods in Neuroendocrinology examines the questions being asked in the field, including what problems need to be solved and what the limits of interpretation are. The experimental approaches described in this book merge endocrine and neuroscientific research and offer insight into the many disciplines involved. Much more than a "cookbook", this is a resource for every researcher who wants to pursue new areas of inquiry in the science of neuroendocrinology.
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Inhaltsangabe
Summary: (1) What are the cellular and molecular mechanisms responsible for the synthesis and secretion of hormones? (2) How do other cells influence neuroendocrine control mechanisms? (3) How do hormones signal their neuroendocrine regulatory neurons? (4) How can we use hormones as indicators of specific changes in neuroendocrine control sites in the brain? Studying morphology of the immortalized hypothalamic LHRH neurons.- Assessment of neuropeptide receptor gene expression using reverse transcriptase polymerase chain reaction.- Identification of biotinylated ligands on specific target cells in the pituitary.- Steroid hormone receptors and the assessment of feedback sensitivity.- In vitro approaches to neuroendocrinology.- Intracellular recording from hypothalamic cells that regulate neuroendocrine function.- Application of in vivo electrophysiological approaches to the study of neuroendocrine systems.- Antisense oligonucleotides.
Summary: (1) What are the cellular and molecular mechanisms responsible for the synthesis and secretion of hormones? (2) How do other cells influence neuroendocrine control mechanisms? (3) How do hormones signal their neuroendocrine regulatory neurons? (4) How can we use hormones as indicators of specific changes in neuroendocrine control sites in the brain? Studying morphology of the immortalized hypothalamic LHRH neurons.- Assessment of neuropeptide receptor gene expression using reverse transcriptase polymerase chain reaction.- Identification of biotinylated ligands on specific target cells in the pituitary.- Steroid hormone receptors and the assessment of feedback sensitivity.- In vitro approaches to neuroendocrinology.- Intracellular recording from hypothalamic cells that regulate neuroendocrine function.- Application of in vivo electrophysiological approaches to the study of neuroendocrine systems.- Antisense oligonucleotides.
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