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Second Generation Multiplex is a DNA profiling systems used in the United Kingdom to set up the UK National DNA Database in 1995. It is manufactured by ABI (Applied Biosystems). It contains primers for the following STR (Short Tandem Repeat) loci. VWA (HUMVWF31/A), D8 (D8S1179), D21 (D21S11), D18 (D18S51), THO (HUMTHO1), FGA (HUMFIBRA). Also contains primers for the Amelogenin sex indicating test.